Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays.

TitleCoupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays.
Publication TypeJournal Article
Year of Publication2015
AuthorsIngale J, Wyatt RT
JournalBio Protoc
Volume5
Issue20
Date Published10/05/2015
ISSN2331-8325
Abstract

Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process. Fine mapping of epitope specific antibodies is difficult when they are elicited at relatively low levels. In our recent study focused at developing an HIV-1 vaccine, we immunized rabbits with hyperglycosylated stable core immunogens, which were designed using high resolution structural information to elicit antibodies against the primary receptor-binding, CD4-binding site on HIV-1 gp120. Using a solid phase adsorption assay, we could map the serum antibodies to the conserved CD4-binding site, a known broadly neutralizing determinant on exterior envelope glycoprotein, gp120.

Alternate JournalBio Protoc
PubMed ID26855964
PubMed Central IDPMC4737960
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
CHAVI-ID: 
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