|Title||Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Ellebedy AH, Jackson KJL, Kissick HT, Nakaya HI, Davis CW, Roskin KM, McElroy AK, Oshansky CM, Elbein R, Thomas S, Lyon GM, Spiropoulou CF, Mehta AK, Thomas PG, Boyd SD, Ahmed R|
Antigen-specific B cells bifurcate into antibody-secreting cells (ASCs) and memory B cells (MBCs) after infection or vaccination. ASCs (plasmablasts) have been extensively studied in humans, but less is known about B cells that become activated but do not differentiate into plasmablasts. Here we have defined the phenotype and transcriptional program of a subset of antigen-specific B cells, which we have called 'activated B cells' (ABCs), that were distinct from ASCs and were committed to the MBC lineage. We detected ABCs in humans after infection with Ebola virus or influenza virus and also after vaccination. By simultaneously analyzing antigen-specific ASCs and ABCs in human blood after vaccination against influenza virus, we investigated the clonal overlap and extent of somatic hypermutation (SHM) in the ASC (effector) and ABC (memory) lineages. Longitudinal tracking of vaccination-induced hemagglutinin (HA)-specific clones revealed no overall increase in SHM over time, which suggested that repeated annual immunization might have limitations in enhancing the quality of influenza-virus-specific antibody.
|Alternate Journal||Nat. Immunol.|
|PubMed Central ID||PMC5054979|
|Grant List||HHSN266200700006C / AI / NIAID NIH HHS / United States |
UM1 AI100663 / AI / NIAID NIH HHS / United States
T32 AI074492 / AI / NIAID NIH HHS / United States
U19 AI117891 / AI / NIAID NIH HHS / United States
P01 AI097092 / AI / NIAID NIH HHS / United States
U01 AI104342 / AI / NIAID NIH HHS / United States
Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.