Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.

TitleDefining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.
Publication TypeJournal Article
Year of Publication2016
AuthorsEllebedy AH, Jackson KJL, Kissick HT, Nakaya HI, Davis CW, Roskin KM, McElroy AK, Oshansky CM, Elbein R, Thomas S, Lyon GM, Spiropoulou CF, Mehta AK, Thomas PG, Boyd SD, Ahmed R
JournalNat Immunol
Volume17
Issue10
Pagination1226-34
Date Published10/01/2016
ISSN1529-2916
Abstract

Antigen-specific B cells bifurcate into antibody-secreting cells (ASCs) and memory B cells (MBCs) after infection or vaccination. ASCs (plasmablasts) have been extensively studied in humans, but less is known about B cells that become activated but do not differentiate into plasmablasts. Here we have defined the phenotype and transcriptional program of a subset of antigen-specific B cells, which we have called 'activated B cells' (ABCs), that were distinct from ASCs and were committed to the MBC lineage. We detected ABCs in humans after infection with Ebola virus or influenza virus and also after vaccination. By simultaneously analyzing antigen-specific ASCs and ABCs in human blood after vaccination against influenza virus, we investigated the clonal overlap and extent of somatic hypermutation (SHM) in the ASC (effector) and ABC (memory) lineages. Longitudinal tracking of vaccination-induced hemagglutinin (HA)-specific clones revealed no overall increase in SHM over time, which suggested that repeated annual immunization might have limitations in enhancing the quality of influenza-virus-specific antibody.

DOI10.1038/ni.3533
Alternate JournalNat. Immunol.
PubMed ID27525369
PubMed Central IDPMC5054979
Grant ListHHSN266200700006C / AI / NIAID NIH HHS / United States
UM1 AI100663 / AI / NIAID NIH HHS / United States
T32 AI074492 / AI / NIAID NIH HHS / United States
U19 AI117891 / AI / NIAID NIH HHS / United States
P01 AI097092 / AI / NIAID NIH HHS / United States
U01 AI104342 / AI / NIAID NIH HHS / United States
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