Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.

TitleDetermination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.
Publication TypeJournal Article
Year of Publication2015
AuthorsBitto D, Harvey DJ, Halldorsson S, Doores KJ, Pritchard LK, Huiskonen JT, Bowden TA, Crispin M
JournalMethods Mol Biol
Volume1331
Pagination93-121
Date Published2015
ISSN1940-6029
Abstract

Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

DOI10.1007/978-1-4939-2874-3_7
Alternate JournalMethods Mol. Biol.
PubMed ID26169737
Grant List090532 / / Wellcome Trust / United Kingdom
090532/Z/09/Z / / Wellcome Trust / United Kingdom
MR/K024426/1 / / Medical Research Council / United Kingdom
MR/L009528/1 / / Medical Research Council / United Kingdom
UM1 AI100663 / AI / NIAID NIH HHS / United States
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