Engineering and Characterization of a Fluorescent Native-Like HIV-1 Envelope Glycoprotein Trimer.

TitleEngineering and Characterization of a Fluorescent Native-Like HIV-1 Envelope Glycoprotein Trimer.
Publication TypeJournal Article
Year of Publication2015
AuthorsSliepen K, van Montfort T, Ozorowski G, Pritchard LK, Crispin M, Ward AB, Sanders RW
JournalBiomolecules
Volume5
Issue4
Pagination2919-34
Date Published10/23/2015
ISSN2218-273X
Abstract

Generation of a stable, soluble mimic of the HIV-1 envelope glycoprotein (Env) trimer on the virion surface has been considered an important first step for developing a successful HIV-1 vaccine. Recently, a soluble native-like Env trimer (BG505 SOSIP.664) has been described. This protein has facilitated major advances in the HIV-1 vaccine field, since it was the first Env immunogen that induced consistent neutralizing antibodies against a neutralization-resistant (tier 2) virus. Moreover, BG505 SOSIP.664 enabled elucidation of the atomic resolution structure of the Env trimer and facilitated the isolation and characterization of new broadly neutralizing antibodies against HIV-1. Here, we designed and characterized the BG505 SOSIP.664 trimer fused to fluorescent superfolder GFP (sfGFP), a GFP variant that allows efficient folding (BG505 SOSIP.664-sfGFP). Despite the presence of the sfGFP, the Env protein largely retained its morphology, antigenicity, glycan composition, and thermostability. In addition, we show that BG505 SOSIP.664-sfGFP can be used for fluorescence-based assays, such as flow cytometry.

DOI10.3390/biom5042919
Alternate JournalBiomolecules
PubMed ID26512709
Grant ListAI100663 / AI / NIAID NIH HHS / United States
HHSN27201100016C / / PHS HHS / United States
P01 AI082362 / AI / NIAID NIH HHS / United States
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