Full-Length HIV-1 Immunogens Induce Greater Magnitude and Comparable Breadth of T Lymphocyte Responses to Conserved HIV-1 Regions Compared With Conserved-Region-Only HIV-1 Immunogens in Rhesus Monkeys.

TitleFull-Length HIV-1 Immunogens Induce Greater Magnitude and Comparable Breadth of T Lymphocyte Responses to Conserved HIV-1 Regions Compared With Conserved-Region-Only HIV-1 Immunogens in Rhesus Monkeys.
Publication TypeJournal Article
Year of Publication2012
AuthorsStephenson KE, Sanmiguel A, Simmons NL, Smith K, Lewis MG, Szinger JJ, Korber B, Barouch DH
JournalJ Virol
Date Published2012 Aug 15
ISSN1098-5514
Abstract

A global HIV-1 vaccine will likely need to induce immune responses against conserved HIV-1 regions to contend with the profound genetic diversity of HIV-1. Here we evaluated the capacity of immunogens consisting of only highly conserved HIV-1 sequences that are aimed at focusing cellular immune responses on these potentially critical regions. We assessed in rhesus monkeys the breadth and magnitude of T lymphocyte responses elicited by adenovirus vectors expressing either full-length HIV-1 Gag/Pol/Env immunogens or concatenated immunogens consisting of only highly conserved HIV-1 sequences. Surprisingly, we found that the full-length immunogens induced comparable breadth (P=1.0) and greater magnitude (P=0.01) of CD8+ T lymphocyte responses against conserved HIV-1 regions as compared with the conserved-region-only immunogens. Moreover, the full-length immunogens induced 5-fold increased total breadth of HIV-1-specific T lymphocyte responses compared with the conserved-region-only immunogens (P=0.007). These results suggest that full-length HIV-1 immunogens elicit substantially increased magnitude and breadth of cellular immune responses compared with conserved-region-only HIV-1 immunogens, including higher magnitude and comparable breadth of responses against conserved sequences.

DOI10.1128/JVI.01779-12
Alternate JournalJ. Virol.
PubMed ID22896617