|Title||Full-Length HIV-1 Immunogens Induce Greater Magnitude and Comparable Breadth of T Lymphocyte Responses to Conserved HIV-1 Regions Compared With Conserved-Region-Only HIV-1 Immunogens in Rhesus Monkeys.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Stephenson KE, Sanmiguel A, Simmons NL, Smith K, Lewis MG, Szinger JJ, Korber B, Barouch DH|
|Date Published||2012 Aug 15|
A global HIV-1 vaccine will likely need to induce immune responses against conserved HIV-1 regions to contend with the profound genetic diversity of HIV-1. Here we evaluated the capacity of immunogens consisting of only highly conserved HIV-1 sequences that are aimed at focusing cellular immune responses on these potentially critical regions. We assessed in rhesus monkeys the breadth and magnitude of T lymphocyte responses elicited by adenovirus vectors expressing either full-length HIV-1 Gag/Pol/Env immunogens or concatenated immunogens consisting of only highly conserved HIV-1 sequences. Surprisingly, we found that the full-length immunogens induced comparable breadth (P=1.0) and greater magnitude (P=0.01) of CD8+ T lymphocyte responses against conserved HIV-1 regions as compared with the conserved-region-only immunogens. Moreover, the full-length immunogens induced 5-fold increased total breadth of HIV-1-specific T lymphocyte responses compared with the conserved-region-only immunogens (P=0.007). These results suggest that full-length HIV-1 immunogens elicit substantially increased magnitude and breadth of cellular immune responses compared with conserved-region-only HIV-1 immunogens, including higher magnitude and comparable breadth of responses against conserved sequences.
|Alternate Journal||J. Virol.|