Harnessing post-translational modifications for next-generation HIV immunogens.

TitleHarnessing post-translational modifications for next-generation HIV immunogens.
Publication TypeJournal Article
Year of Publication2018
AuthorsAllen JD, Sanders RW, Doores KJ, Crispin M
JournalBiochem Soc Trans
Volume46
Issue3
Pagination691-698
Date Published06/19/2018
ISSN1470-8752
Abstract

The extensive post-translational modifications of the envelope spikes of the human immunodeficiency virus (HIV) present considerable challenges and opportunities for HIV vaccine design. These oligomeric glycoproteins typically have over 30 disulfide bonds and around a 100 N-linked glycosylation sites, and are functionally dependent on protease cleavage within the secretory system. The resulting mature structure adopts a compact fold with the vast majority of its surface obscured by a protective shield of glycans which can be targeted by broadly neutralizing antibodies (bnAbs). Despite the notorious heterogeneity of glycosylation, rare B-cell lineages can evolve to utilize and cope with viral glycan diversity, and these structures therefore present promising targets for vaccine design. The latest generation of recombinant envelope spike mimetics contains re-engineered post-translational modifications to present stable antigens to guide the development of bnAbs by vaccination.

DOI10.1042/BST20170394
Alternate JournalBiochem. Soc. Trans.
PubMed ID29784645
PubMed Central IDPMC6013407
Grant ListP01 AI110657 / AI / NIAID NIH HHS / United States
UM1 AI100663 / AI / NIAID NIH HHS / United States
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