HIV-1 envelope trimer elicits more potent neutralizing antibody responses than monomeric gp120.

TitleHIV-1 envelope trimer elicits more potent neutralizing antibody responses than monomeric gp120.
Publication TypeJournal Article
Year of Publication2012
AuthorsKovacs JM, Nkolola JP, Peng H, Cheung A, Perry J, Miller CA, Seaman MS, Barouch DH, Chen B
JournalProc Natl Acad Sci U S A
Volume109
Issue30
Pagination12111-6
Date Published2012 Jul 24
ISSN1091-6490
Abstract

HIV-1 envelope glycoprotein is the primary target for HIV-1-specific antibodies. The native HIV-1 envelope spike on the virion surface is a trimer, but trimeric gp140 and monomeric gp120 currently are believed to induce comparable immune responses. Indeed, most studies on the immunogenicity of HIV-1 envelope oligomers have revealed only marginal improvement over monomers. We report here that suitably prepared envelope trimers have nearly all the antigenic properties expected for native viral spikes. These stable, rigorously homogenous trimers have antigenic properties markedly different from those of monomeric gp120s derived from the same sequences, and they induce potent neutralizing antibody responses for a cross-clade set of tier 1 and tier 2 viruses with titers substantially higher than those elicited by the corresponding gp120 monomers. These results, which demonstrate that there are relevant immunologic differences between monomers and high-quality envelope trimers, have important implications for HIV-1 vaccine development.

DOI10.1073/pnas.1204533109
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID22773820