Identification and specificity of broadly neutralizing antibodies against HIV.

TitleIdentification and specificity of broadly neutralizing antibodies against HIV.
Publication TypeJournal Article
Year of Publication2017
AuthorsMcCoy LE, Burton DR
JournalImmunol Rev
Volume275
Issue1
Pagination11-20
Date Published01/01/2017
ISSN1600-065X
Abstract

Beginning in 2009, studies of the humoral responses of HIV-positive individuals have led to the identification of scores, if not hundreds, of antibodies that are both broadly reactive and potently neutralizing. This development has provided renewed impetus toward an HIV vaccine and led directly to the development of novel immunogens. Advances in identification of donors with the most potent and broad anti-HIV serum neutralizing responses were crucial in this effort. Equally, development of methods for the rapid generation of human antibodies from these donors was pivotal. Primarily these methods comprise single B-cell culture coupled to high-throughput neutralization screening and flow cytometry-based sorting of single B cells using HIV envelope protein baits. In this review, the advantages and disadvantages of these methodologies are discussed in the context of the specificities targeted by individual antibodies and the need for further improvements to evaluate HIV vaccine candidates.

DOI10.1111/imr.12484
Alternate JournalImmunol. Rev.
PubMed ID28133814
PubMed Central IDPMC5299474
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
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