Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160Δc(BaL.).

TitleIsolation of HIV-1-reactive antibodies using cell surface-expressed gp160Δc(BaL.).
Publication TypeJournal Article
Year of Publication2013
AuthorsGaebler C, Gruell H, Velinzon K, Scheid JF, Nussenzweig MC, Klein F
JournalJ Immunol Methods
Volume397
Issue1-2
Pagination47-54
Date Published11/29/2013
ISSN1872-7905
Abstract

Significant efforts have been made to identify HIV-1 neutralizing antibodies because they are considered to be critical to the design of an effective HIV-1 vaccine. Although soluble HIV-1 envelope proteins can be used for this purpose, these reagents differ from membrane-anchored HIV-1 envelope spike in a number of important ways and display only a subset of its native epitopes. Consistent with this, some broadly neutralizing antibodies preferentially bind cell surface-expressed HIV-1 envelope, but not the soluble protein. Here we report the details of a new method for isolating anti-HIV-1 specific B cells based on capturing cells that produce antibodies to cell surface-expressed gp160Δc(BaL). While this method is far less efficient than sorting with soluble envelope proteins, it isolated broadly neutralizing anti-HIV-1 antibodies that bind cell surface-expressed gp160Δc(BaL) but not soluble envelope proteins.

DOI10.1016/j.jim.2013.09.003
Alternate JournalJ. Immunol. Methods
PubMed ID24041474
Grant ListP01 AI081677 / AI / NIAID NIH HHS / United States
UM1 AI100663 / AI / NIAID NIH HHS / United States
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