|Title||Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160Δc(BaL.).|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Gaebler C, Gruell H, Velinzon K, Scheid JF, Nussenzweig MC, Klein F|
|Journal||J Immunol Methods|
Significant efforts have been made to identify HIV-1 neutralizing antibodies because they are considered to be critical to the design of an effective HIV-1 vaccine. Although soluble HIV-1 envelope proteins can be used for this purpose, these reagents differ from membrane-anchored HIV-1 envelope spike in a number of important ways and display only a subset of its native epitopes. Consistent with this, some broadly neutralizing antibodies preferentially bind cell surface-expressed HIV-1 envelope, but not the soluble protein. Here we report the details of a new method for isolating anti-HIV-1 specific B cells based on capturing cells that produce antibodies to cell surface-expressed gp160Δc(BaL). While this method is far less efficient than sorting with soluble envelope proteins, it isolated broadly neutralizing anti-HIV-1 antibodies that bind cell surface-expressed gp160Δc(BaL) but not soluble envelope proteins.
|Alternate Journal||J. Immunol. Methods|
|Grant List||P01 AI081677 / AI / NIAID NIH HHS / United States |
UM1 AI100663 / AI / NIAID NIH HHS / United States
Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160Δc(BaL.).