Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.

TitleIsomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.
Publication TypeJournal Article
Year of Publication2018
AuthorsHarvey DJ, Seabright GE, Vasiljevic S, Crispin M, Struwe WB
JournalJ Am Soc Mass Spectrom
Volume29
Issue5
Pagination972-988
Date Published05/01/2018
ISSN1879-1123
Abstract

Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans. Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers. For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on ManGlcNAc isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures. Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra. Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated. Graphical Abstract ᅟ.

DOI10.1007/s13361-018-1890-5
Alternate JournalJ Am Soc Mass Spectrom
PubMed ID29508223
PubMed Central IDPMC5940726
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
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