Kinetic Analysis of Monoclonal Antibody Binding to HIV-1 gp120-derived Hyperglycosylated Cores.

TitleKinetic Analysis of Monoclonal Antibody Binding to HIV-1 gp120-derived Hyperglycosylated Cores.
Publication TypeJournal Article
Year of Publication2015
AuthorsIngale J, Wyatt RT
JournalBio Protoc
Volume5
Issue20
Date Published10/05/2015
ISSN2331-8325
Abstract

Kinetic analysis of antibodies is one of the important study for characterization of antibodies and screening of ligands. In our recent study(1), we compared the antigenic profiles and binding characteristics of four HIV-1 envelope glycoprotein (Env) core immunogens using multiple monoclonal antibodies by Bio-Layer Light Interferometry (BLI). This technology enables real-time analysis of interactions on the surface of a fiber optic biosensor by accurately measuring kinetic constants such as Ka, Kd, and KD in a 96-well format.

Alternate JournalBio Protoc
PubMed ID26855965
PubMed Central IDPMC4737964
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
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