The microanatomic segregation of selection by apoptosis in the germinal center.

TitleThe microanatomic segregation of selection by apoptosis in the germinal center.
Publication TypeJournal Article
Year of Publication2017
AuthorsMayer CT, Gazumyan A, Kara EE, Gitlin AD, Golijanin J, Viant C, Pai J, Oliveira TY, Wang Q, Escolano A, Medina-Ramírez M, Sanders RW, Nussenzweig MC
JournalScience
Volume358
Issue6360
Date Published10/13/2017
ISSN1095-9203
Abstract

B cells undergo rapid cell division and affinity maturation in anatomically distinct sites in lymphoid organs called germinal centers (GCs). Homeostasis is maintained in part by B cell apoptosis. However, the precise contribution of apoptosis to GC biology and selection is not well defined. We developed apoptosis-indicator mice and used them to visualize, purify, and characterize dying GC B cells. Apoptosis is prevalent in the GC, with up to half of all GC B cells dying every 6 hours. Moreover, programmed cell death is differentially regulated in the light zone and the dark zone: Light-zone B cells die by default if they are not positively selected, whereas dark-zone cells die when their antigen receptors are damaged by activation-induced cytidine deaminase.

DOI10.1126/science.aao2602
Alternate JournalScience
PubMed ID28935768
Grant ListT32 GM007739 / GM / NIGMS NIH HHS / United States
F30 AI109903 / AI / NIAID NIH HHS / United States
UM1 AI100663 / AI / NIAID NIH HHS / United States
P01 AI100148 / AI / NIAID NIH HHS / United States
R01 AI037526 / AI / NIAID NIH HHS / United States
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