|Title||The microanatomic segregation of selection by apoptosis in the germinal center.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Mayer CT, Gazumyan A, Kara EE, Gitlin AD, Golijanin J, Viant C, Pai J, Oliveira TY, Wang Q, Escolano A, Medina-Ramírez M, Sanders RW, Nussenzweig MC|
B cells undergo rapid cell division and affinity maturation in anatomically distinct sites in lymphoid organs called germinal centers (GCs). Homeostasis is maintained in part by B cell apoptosis. However, the precise contribution of apoptosis to GC biology and selection is not well defined. We developed apoptosis-indicator mice and used them to visualize, purify, and characterize dying GC B cells. Apoptosis is prevalent in the GC, with up to half of all GC B cells dying every 6 hours. Moreover, programmed cell death is differentially regulated in the light zone and the dark zone: Light-zone B cells die by default if they are not positively selected, whereas dark-zone cells die when their antigen receptors are damaged by activation-induced cytidine deaminase.
|Grant List||T32 GM007739 / GM / NIGMS NIH HHS / United States |
F30 AI109903 / AI / NIAID NIH HHS / United States
UM1 AI100663 / AI / NIAID NIH HHS / United States
P01 AI100148 / AI / NIAID NIH HHS / United States
R01 AI037526 / AI / NIAID NIH HHS / United States
The microanatomic segregation of selection by apoptosis in the germinal center.