Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques.

TitlePassive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques.
Publication TypeJournal Article
Year of Publication2014
AuthorsShingai M, Donau OK, Plishka RJ, Buckler-White A, Mascola JR, Nabel GJ, Nason MC, Montefiori D, Moldt B, Poignard P, Diskin R, Bjorkman PJ, Eckhaus MA, Klein F, Mouquet H, Lorenzi JCesar Cetr, Gazumyan A, Burton DR, Nussenzweig MC, Martin MA, Nishimura Y
JournalJ Exp Med
Volume211
Issue10
Pagination2061-74
Date Published09/22/2014
ISSN1540-9538
KeywordsAnimals, Antibodies, Monoclonal, Antibodies, Neutralizing, DNA Primers, HIV, HIV Antibodies, Humans, Lentivirus Infections, Macaca mulatta, Mutagenesis, Neutralization Tests, Regression Analysis, Simian immunodeficiency virus, Vaccination
Abstract

It is widely appreciated that effective human vaccines directed against viral pathogens elicit neutralizing antibodies (NAbs). The passive transfer of anti-HIV-1 NAbs conferring sterilizing immunity to macaques has been used to determine the plasma neutralization titers, which must be present at the time of exposure, to prevent acquisition of SIV/HIV chimeric virus (SHIV) infections. We administered five recently isolated potent and broadly acting anti-HIV neutralizing monoclonal antibodies (mAbs) to rhesus macaques and challenged them intrarectally 24 h later with either of two different R5-tropic SHIVs. By combining the results obtained from 60 challenged animals, we determined that the protective neutralization titer in plasma preventing virus infection in 50% of the exposed monkeys was relatively modest (∼1:100) and potentially achievable by vaccination.

DOI10.1084/jem.20132494
Alternate JournalJ. Exp. Med.
PubMed ID25155019
PubMed Central IDPMC4172223
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
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