Protection of Humanized Mice From Repeated Intravaginal HIV Challenge by Passive Immunization: A Model for Studying the Efficacy of Neutralizing Antibodies In Vivo.

TitleProtection of Humanized Mice From Repeated Intravaginal HIV Challenge by Passive Immunization: A Model for Studying the Efficacy of Neutralizing Antibodies In Vivo.
Publication TypeJournal Article
Year of Publication2016
AuthorsDeruaz M, Moldt B, Le KM, Power KA, Vrbanac VD, Tanno S, Ghebremichael MS, Allen TM, Tager AM, Burton DR, Luster AD
JournalJ Infect Dis
Volume214
Issue4
Pagination612-6
Date Published08/15/2016
ISSN1537-6613
Abstract

Humanized mice reconstituted with a human immune system can be mucosally infected with human immunodeficiency virus (HIV), opening up the possibility of studying HIV transmission in a small-animal model. Here we report that passive immunization with the broadly neutralizing antibody b12 protected humanized mice against repetitive intravaginal infection in a dose-dependent manner. In addition, treatment with the antibody PGT126, which is more potent in vitro, was more efficacious in vivo and provided sterilizing protection. Our results demonstrate that humanized mice can be used as a small-animal model to study the efficacy and mechanism of broadly neutralizing antibody protection against HIV acquisition.

DOI10.1093/infdis/jiw203
Alternate JournalJ. Infect. Dis.
PubMed ID27357340
PubMed Central IDPMC4957443
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
P01 AI078897 / AI / NIAID NIH HHS / United States
P01 AI104715 / AI / NIAID NIH HHS / United States
R01 AI033292 / AI / NIAID NIH HHS / United States
P30 AI060354 / AI / NIAID NIH HHS / United States
CHAVI-ID: 
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