Structural analysis of glycoproteins: building N-linked glycans with Coot.

TitleStructural analysis of glycoproteins: building N-linked glycans with Coot.
Publication TypeJournal Article
Year of Publication2018
AuthorsEmsley P, Crispin M
JournalActa Crystallogr D Struct Biol
Volume74
IssuePt 4
Pagination256-263
Date Published04/01/2018
ISSN2059-7983
Abstract

Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level. The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans. The model is built by the addition of monosaccharides, placed by variation of internal coordinates. The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints. It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.

DOI10.1107/S2059798318005119
Alternate JournalActa Crystallogr D Struct Biol
PubMed ID29652253
PubMed Central IDPMC5892875
Grant ListMC_UP_A025_1012 / / Medical Research Council / United Kingdom
1UM1AI100663 / / Scripps Howard Foundation /
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