|Title||Structural analysis of glycoproteins: building N-linked glycans with Coot.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Emsley P, Crispin M|
|Journal||Acta Crystallogr D Struct Biol|
Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level. The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans. The model is built by the addition of monosaccharides, placed by variation of internal coordinates. The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints. It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
|Alternate Journal||Acta Crystallogr D Struct Biol|
|PubMed Central ID||PMC5892875|
|Grant List||MC_UP_A025_1012 / / Medical Research Council / United Kingdom |
1UM1AI100663 / / Scripps Howard Foundation /
Structural analysis of glycoproteins: building N-linked glycans with Coot.