Structure of the Lassa virus glycan shield provides a model for immunological resistance.

TitleStructure of the Lassa virus glycan shield provides a model for immunological resistance.
Publication TypeJournal Article
Year of Publication2018
AuthorsWatanabe Y, Raghwani J, Allen JD, Seabright GE, Li S, Moser F, Huiskonen JT, Strecker T, Bowden TA, Crispin M
JournalProc Natl Acad Sci U S A
Volume115
Issue28
Pagination7320-7325
Date Published07/10/2018
ISSN1091-6490
Abstract

Lassa virus is an Old World arenavirus endemic to West Africa that causes severe hemorrhagic fever. Vaccine development has focused on the envelope glycoprotein complex (GPC) that extends from the virion envelope. The often inadequate antibody immune response elicited by both vaccine and natural infection has been, in part, attributed to the abundance of N-linked glycosylation on the GPC. Here, using a virus-like-particle system that presents Lassa virus GPC in a native-like context, we determine the composite population of each of the N-linked glycosylation sites presented on the trimeric GPC spike. Our analysis reveals the presence of underprocessed oligomannose-type glycans, which form punctuated clusters that obscure the proteinous surface of both the GP1 attachment and GP2 fusion glycoprotein subunits of the Lassa virus GPC. These oligomannose clusters are seemingly derived as a result of sterically reduced accessibility to glycan processing enzymes, and limited amino acid diversification around these sites supports their role protecting against the humoral immune response. Combined, our data provide a structure-based blueprint for understanding how glycans render the glycoprotein spikes of Lassa virus and other Old World arenaviruses immunologically resistant targets.

DOI10.1073/pnas.1803990115
Alternate JournalProc Natl Acad Sci U S A
PubMed ID29941589
PubMed Central IDPMC6048489
Grant List / / Wellcome Trust / United Kingdom
UM1 AI100663 / AI / NIAID NIH HHS / United States
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