|Title||T cell help controls the speed of the cell cycle in germinal center B cells.|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Gitlin AD, Mayer CT, Oliveira TY, Shulman, iv Z, Jones MJK, Koren A, Nussenzweig MC|
The germinal center (GC) is a microanatomical compartment wherein high-affinity antibody-producing B cells are selectively expanded. B cells proliferate and mutate their antibody genes in the dark zone (DZ) of the GC and are then selected by T cells in the light zone (LZ) on the basis of affinity. Here, we show that T cell help regulates the speed of cell cycle phase transitions and DNA replication of GC B cells. Genome sequencing and single-molecule analyses revealed that T cell help shortens S phase by regulating replication fork progression while preserving the relative order of replication origin activation. Thus, high-affinity GC B cells are selected by a mechanism that involves prolonged dwell time in the DZ where selected cells undergo accelerated cell cycles.
|Grant List||UM1 AI100663 / AI / NIAID NIH HHS / United States|
T cell help controls the speed of the cell cycle in germinal center B cells.