T follicular helper cell dynamics in germinal centers.

TitleT follicular helper cell dynamics in germinal centers.
Publication TypeJournal Article
Year of Publication2013
AuthorsShulman, iv Z, Gitlin AD, Targ S, Jankovic M, Pasqual G, Nussenzweig MC, Victora GD
JournalScience
Volume341
Issue6146
Pagination673-7
Date Published08/09/2013
ISSN1095-9203
KeywordsAnimals, Antigenic Variation, B-Lymphocytes, Clone Cells, Germinal Center, Mice, T-Lymphocytes, Helper-Inducer
Abstract

T follicular helper (T(FH)) cells are a specialized subset of effector T cells that provide help to and thereby select high-affinity B cells in germinal centers (GCs). To examine the dynamic behavior of T(FH) cells in GCs in mice, we used two-photon microscopy in combination with a photoactivatable fluorescent reporter. Unlike GC B cells, which are clonally restricted, T(FH) cells distributed among all GCs in lymph nodes and continually emigrated into the follicle and neighboring GCs. Moreover, newly activated T(FH) cells invaded preexisting GCs, where they contributed to B cell selection and plasmablast differentiation. Our data suggest that the dynamic exchange of T(FH) cells between GCs ensures maximal diversification of T cell help and that their ability to enter ongoing GCs accommodates antigenic variation during the immune response.

DOI10.1126/science.1241680
Alternate JournalScience
PubMed ID23887872
Grant List5DP5OD012146-02 / OD / NIH HHS / United States
AI037526-19 / AI / NIAID NIH HHS / United States
AI072529-06 / AI / NIAID NIH HHS / United States
AI100663-01 / AI / NIAID NIH HHS / United States
T32GM07739 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
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