Tools for Visualizing HIV in Cure Research.

TitleTools for Visualizing HIV in Cure Research.
Publication TypeJournal Article
Year of Publication2018
AuthorsNiessl J, Baxter AE, Kaufmann DE
JournalCurr HIV/AIDS Rep
Date Published01/27/2018
ISSN1548-3576
Abstract

PURPOSE OF REVIEW: The long-lived HIV reservoir remains a major obstacle for an HIV cure. Current techniques to analyze this reservoir are generally population-based. We highlight recent developments in methods visualizing HIV, which offer a different, complementary view, and provide indispensable information for cure strategy development.

RECENT FINDINGS: Recent advances in fluorescence in situ hybridization techniques enabled key developments in reservoir visualization. Flow cytometric detection of HIV mRNAs, concurrently with proteins, provides a high-throughput approach to study the reservoir on a single-cell level. On a tissue level, key spatial information can be obtained detecting viral RNA and DNA in situ by fluorescence microscopy. At total-body level, advancements in non-invasive immuno-positron emission tomography (PET) detection of HIV proteins may allow an encompassing view of HIV reservoir sites. HIV imaging approaches provide important, complementary information regarding the size, phenotype, and localization of the HIV reservoir. Visualizing the reservoir may contribute to the design, assessment, and monitoring of HIV cure strategies in vitro and in vivo.

DOI10.1007/s11904-018-0376-1
Alternate JournalCurr HIV/AIDS Rep
PubMed ID29374858
Grant ListRO1 HL-092565 / / National Institutes of Health / United States
UM1AI100663 / / National Institute of Allergy and Infectious Diseases / United States
377124 / / Canadian Institutes of Health Research / Canada
152536 / / CHIR / United States
31035 / / FRQS Senior Research Scholar Award / United States
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