Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G.

TitleTrimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G.
Publication TypeJournal Article
Year of Publication2016
AuthorsStewart-Jones GBE, Soto C, Lemmin T, Chuang G-Y, Druz A, Kong R, Thomas PV, Wagh K, Zhou T, Behrens A-J, Bylund T, Choi CW, Davison JR, Georgiev IS, M Joyce G, Kwon Y D, Pancera M, Taft J, Yang Y, Zhang B, Shivatare SS, Shivatare VS, Lee C-CD, Wu C-Y, Bewley CA, Burton DR, Koff WC, Connors M, Crispin M, Baxa U, Korber BT, Wong C-H, Mascola JR, Kwong PD
JournalCell
Volume165
Issue4
Pagination813-26
Date Published05/05/2016
ISSN1097-4172
KeywordsAntibodies, Neutralizing, Antibodies, Viral, Crystallography, X-Ray, env Gene Products, Human Immunodeficiency Virus, Glycosylation, HIV-1, Immune Evasion, Models, Molecular, Molecular Dynamics Simulation, Polysaccharides
Abstract

The HIV-1-envelope (Env) trimer is covered by a glycan shield of ∼90 N-linked oligosaccharides, which comprises roughly half its mass and is a key component of HIV evasion from humoral immunity. To understand how antibodies can overcome the barriers imposed by the glycan shield, we crystallized fully glycosylated Env trimers from clades A, B, and G, visualizing the shield at 3.4-3.7 Å resolution. These structures reveal the HIV-1-glycan shield to comprise a network of interlocking oligosaccharides, substantially ordered by glycan crowding, that encase the protein component of Env and enable HIV-1 to avoid most antibody-mediated neutralization. The revealed features delineate a taxonomy of N-linked glycan-glycan interactions. Crowded and dispersed glycans are differently ordered, conserved, processed, and recognized by antibody. The structures, along with glycan-array binding and molecular dynamics, reveal a diversity in oligosaccharide affinity and a requirement for accommodating glycans among known broadly neutralizing antibodies that target the glycan-shielded trimer.

DOI10.1016/j.cell.2016.04.010
Alternate JournalCell
PubMed ID27114034
Grant ListUM1 AI100663 / AI / NIAID NIH HHS / United States
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