Zika Virus Infects Human Placental Macrophages.

TitleZika Virus Infects Human Placental Macrophages.
Publication TypeJournal Article
Year of Publication2016
AuthorsQuicke KM, Bowen JR, Johnson EL, McDonald CE, Ma H, O'Neal JT, Rajakumar A, Wrammert J, Rimawi BH, Pulendran B, Schinazi RF, Chakraborty R, Suthar MS
JournalCell Host Microbe
Volume20
Issue1
Pagination83-90
Date Published2016 Jul 13
ISSN1934-6069
Abstract

The recent Zika virus (ZIKV) outbreak in Brazil has been directly linked to increased cases of microcephaly in newborns. Current evidence indicates that ZIKV is transmitted vertically from mother to fetus. However, the mechanism of intrauterine transmission and the cell types involved remain unknown. We demonstrate that the contemporary ZIKV strain PRVABC59 (PR 2015) infects and replicates in primary human placental macrophages, called Hofbauer cells, and to a lesser extent in cytotrophoblasts, isolated from villous tissue of full-term placentae. Viral replication coincides with induction of type I interferon (IFN), pro-inflammatory cytokines, and antiviral gene expression, but with minimal cell death. Our results suggest a mechanism for intrauterine transmission in which ZIKV gains access to the fetal compartment by directly infecting placental cells and disrupting the placental barrier.

DOI10.1016/j.chom.2016.05.015
Alternate JournalCell Host Microbe
PubMed ID27247001
PubMed Central IDPMC5166429
Grant ListR37 AI048638 / AI / NIAID NIH HHS / United States
P30 AI050409 / AI / NIAID NIH HHS / United States
U19 AI090023 / AI / NIAID NIH HHS / United States
U19 AI083019 / AI / NIAID NIH HHS / United States
R56 AI110516 / AI / NIAID NIH HHS / United States
R21 AI113485 / AI / NIAID NIH HHS / United States
U19 AI057266 / AI / NIAID NIH HHS / United States
R37 DK057665 / DK / NIDDK NIH HHS / United States
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